About Us

LASEC Transgenic and Cryogenic Facility (LTCF) is embedded in LASEC to provide cryopreservation, recovery and rederivation of mouse lines using a wide range of Assisted Reproductive Technologies (ARTs), facilitating the creation and management of genetically engineered mouse models for scientific research. Tailor-made strain services including strain rescue and speed expansion are also available upon request.

Contact information
Heidi NG ​
Laboratory Manager & Embryologist 
M +852 6128 1647 | E shukhanng.heidi@cuhk.edu.hk 
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​Reproductive Sciences
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Cryopreservation & Storage

​Cryopreservation archives mouse strains for long-term storage and protects them against genetic drift and accidental loss.

Import & Cryorecovery

Cryorecovery is used to re-establish a line from frozen stock, either from the LTCF’s archive or another repository. 

Rederivation

Embryo transfer is an effective method to rederive mice known to be infected with viruses, bacteria, and parasites.

Strain Rescue

​Our custom-made service can rescue your endangered mouse strain from extinction due to breeding cessation or to health issues.

Speed Colony Expansion

Speed expansion service via in vitro fertilization (IVF) is able to rapidly expand your mouse colony for production of experimental cohort.

Research Collaboration

We look forward to working with you to achieve your research goals in the near future!​

Genome Engineering Technologies
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Whether you need a tailor-made model or already have starting materials for microinjection, LTCF can help.

Pronuclear/ Cytoplasmic Microinjection

This technique provides an efficient means of introducing foreign DNA, RNA, and ribonucleoproteins into the mouse genome for production of genetically engineered mouse model (GEMM).
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Genetically Engineered Model (GEM) Generation

We are pleased to announce that LTCF has started to provide custom-designed CRISPR/Cas9-mediated GEM generation services for CUHK users. Capabilities of genetic modifications for PHASE ONE ​include gene knockout, small deletion, point mutation (SNP), and conditional knockouts through the insertion of loxP sites.